If patients were HPV DNA positive for at least one of the 5 HR HPV types (HPV 16, 18, 31, 33, 45) they were analyzed for the expression of viral oncogenes E6 and E7, looking for the mRNA by the NucliSens EasyQ HPV assay method (bioMerieux). Analyses of the samples were performed by the Virology Laboratory at the University Hospital of Catania After long-term follow-up, the future risk of HSIL+ in women with a negative HPV E6/E7 mRNA test result was found to be quite low, as was that of women with negative results in DNA-based assays (Reid et al., 2015; Iftner et al., 2019). In this study, HPV E6/E7 mRNA testing showed good performance in population-based cervical cancer screening The proportion of subjects (expressed as percent) without a certain disease (e.g. CIN II or higher severity) who have a NEGATIVE HPV test. E.g., a specificity of 70% means that if 100 women without CIN II or higher severity participate in the screening, 30 will be POSITIVE (false-positive) by the test. Specificity is affected mainly by the test The HR-HPV-negative samples were included in E6/E7 mRNA testing because the study aimed to determine the mRNA test’s clinical characteristics by evaluating and comparing it with the HPV DNA test. Total RNA was extracted from the prepared sample using the miRNeasy Mini Kit and QIAcube robotic workstation (Qiagen, Hilden, Germany) following the *Co-Testing: Cervical cytology plus the HPV co-test is performed on the same date of service. The result of the HPV test is reported regardless if the Pap has a positive or negative result. ** Reflex: Cervical cytology with reflex HPV testing means if the result of the Pap is ASC-US, then the HPV sample is ran by the laboratory. Co-Testing: Use of both the Pap test and human papillomavirus (HPV) test to screen for cervical cancer. Diabetes Mellitus: A condition in which the levels of sugar in the blood are too high. False-Negative: A test result that says you do not have a condition when you do. False-Positive: A test result that says you have a condition when you do not. 3IC7. Previous literature has indicated that TTMV-HPV DNA can be detected in plasma several years before diagnosis; therefore, we defined a true positive test as any positive TTMV-HPV DNA test leading up to a pathologically confirmed recurrence. 15 We considered a negative TTMV-HPV DNA test measured within 3 months of a pathologically confirmed Among the patients who had ASC-H with negative follow-up, 50% had positive HR-HPV results, and 50% had negative HR-HPV results. Conclusions: Among patients with ASC-H, a negative HR-HPV result was found to be an excellent predictor of the absence of HSIL. The results of this pilot study suggested that HPV-DNA testing may serve as a means to HPV testing has been proposed as an adjunctive test to improve the sensitivity and negative predictive value of the Pap smear. At a reduced cost, HPV testing could improve sensitivity with only Screening for infection with high-risk human papillomavirus (HPV) associated with the development of cervical cancer Individual genotyping of HPV-16 and/or HPV-18 if present This testing is intended for use in clinical monitoring and management of patients. It is not intended for use in medical-legal applications. This test is not intended for women who have undergone hysterectomy. This test Overall our findings suggest that DNA methylation-based tests are suitable for the triage of hrHPV-positive women. With the goal to provide a triage test that complements the limited specificity of HPV testing in HPV-based screening, GynTect® may be preferable, due to its higher specificity for CIN2+ or CIN3+ . Women with abnormal Pap test results or a positive HPV test (at least 1 pg of high-risk HPV DNA per milliliter) underwent colposcopy and biopsy, as did a random sample of women with negative tests. Sensitivity and specificity estimates were corrected for verification bias.

hpv dna test negative means